Application

Research Sub CategoryFragment Specific Secondary Antibodies

Goat anti-Human IgM Antibody, Fc5µ, HRP conjugate is an antibody against Human IgM for use in ELISA & WB.

Research CategorySecondary & Control Antibodies

EIA: 1:5,000-1:100,000.
Western blots: 1:5,000-1:200,000.
Immunohistochemistry: 1:500-1:5,000.

Optimal working dilutions must be determined by the end user.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

IgM constitutes about 10% of serum immunoglobulins. IgM is prominent in early immune responses to most antigens and predominates in certain antibody responses such as ′natural′ blood group antibodies. IgM (with IgD) is the major immunoglobulin expressed on the surface of B cells.

Immunogen

Prepared from purified human IgM Fc5µ fragment.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Physical form

0.01M Sodium Phosphate, 0.25M NaCl, pH 7.6 with 15mg/mL BSA. Contains no preservative. Lyophilized.

Specificity

Based on immunoelectrophoresis, the antibody reacts with the Fc5µ portion of the human IgM heavy chain. Does not react with human IgG or IgA, or with the light chains of human immunoglobulins. No reactivity against non-immunoglobulin serum proteins, but the antibody may cross-react with IgM from other species.